Test Detail
Understanding the Test
Interpreting Results
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Written by
Dr. Shreya Gupta
BDS, MDS - Oral and Maxillofacial Pathology
Reviewed by
Dr. Ashish Ranjan
MBBS, MD (Pharmacology)
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Vitamin D Profile (D2, D3 & D Total)

Vitamin D Profile (D2, D3 & D Total) Includes 3 testsView All
You need to provide
Blood
This test is for
Male, Female

Understanding Vitamin D Profile (D2, D3 & D Total)


What is Vitamin D Profile (D2, D3 & D Total)?

The vitamin -D profile measures the levels of vitamin D in your body. This profile offers a Vitamin D3 test, Vitamin D2 test, and Vitamin D test (Total). You may need this test if you have symptoms such as bone pain, muscle weakness, fatigue, loss of appetite,  nausea, and frequent urination. This test is also a useful indicator of osteoporosis (bone weakness), rickets (bone malformation), and osteomalacia(bone softening). Measures such as eating food containing vitamin D and getting more sunlight may help maintain optimal vitamin D levels in your body. 

 

What is Vitamin D Profile (D2, D3 & D Total) used for?

 

 

  • To determine vitamin D deficiency when calcium level is low or a person has symptoms of vitamin D deficiency such as bone malformation in children (rickets), bone weakness, softness, or fracture in adults (osteomalacia).
  • Before starting osteoporosis therapy.
  • To monitor that the treatment is correct for patients who are receiving Vitamin D supplements, calcium, phosphorus, and/or magnesium supplements.
  • To diagnose/monitor problems with parathyroid gland functioning since the parathyroid hormone (PTH) is essential for vitamin D activation.
  • To monitor the health status of individuals with diseases that interfere with fat absorption (e.g., cystic fibrosis and Crohn’s disease) and in patients who have undergone gastric bypass surgery.

 

 

Interpreting Vitamin D Profile (D2, D3 & D Total) results


Interpretations

Vitamin D level below the normal reference range indicates that vitamin D deficiency. This can be because of less sun exposure, dietary deficiency, or due to decreased absorption from the intestine.

Vitamin D level above the normal reference range indicates vitamin D intoxication. This is usually due to excess supplementation of the vitamin.

 

Range:

The normal range of vitamin D Total is measured as nanograms per milliliter (ng/mL) or nmol/L and can vary from lab to lab.

 

Level

Reference range (ng/mL)

Deficiency

<20 ng/mL

Insufficiency 

20-30 ng/mL

Sufficiency

30-100 ng/mL

Toxicity

>100 ng/mL

 

Note:

·       The assay measures both vitamin D2 (Ergocalciferol) and vitamin D3 (Cholecalciferol) metabolites of vitamin D.

·       There can be seasonal variation in 25 (OH) vitamin D level, with values being 40-50% lower in winter than in summer. It is also influenced by sunlight, latitude, skin pigmentation, sunscreen use, and hepatic function.

·       25 (OH) vitamin D levels can vary with age

·       25 (OH) vitamin D level is increased in pregnancy.

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